SECM-based monitoring of tissue growth/differentiation and of biological processes
Scanning Electrochemical Microscopy (SECM) serves as a powerful tool for studying chemical processes at the μm level. The goal of this project is to use the versatile SECM setup for electrochemically monitoring complex biological process using an Ultra microelectrode (UME). There are two approaches that will be undertaken to study biological processes – single cell analysis and tissue analysis.
Monitoring of biological process on single cells
At a single cellular level, one of the simplest measurements that can be performed is monitoring the respiratory activity around a cell (see fig 1 and 2) by following the oxygen concentration changes in the cell vicinity. In the future, other relevant redox species will be studied to elucidate processes occurring in the cell.
Monitoring of biological process on functional microtissues
Microtissues are very good models for understanding cancer and tissue biology. The SECM can serve as a tool to non-invasively monitor chemical activity around these microtissues and also for high-throughput screening assays of drugs on cancer microtissues. Relevant parameters will be targeted for study based on cell type. We have developed a novel platform for large scale producing microtissues and which is compatible with SECM measurements. Devices are fabricated by imprinting arrays of microwells in commercially available Petri dishes (Fig 3), and these are used as platforms to produce tissues (Fig 4).