My Google Scholar profile can be found here and my Research ID is B-7806-2013

Cover Articles

 High Yield, Reproducible and Qausi-automated bilayer Formation in a microfluidic format  Human reproduction  Parallel single-cell analysis microfluidic platform  January 2006
cover electrophoresis Hoon 
 cover tr biotech


  1. Hoon Suk Rho, Yoonsun Yang, Leon WMM Terstappen, Han Gardeniers, Séverine Le Gac*, Pamela Habibović*, Programmable droplet-based Microfluidic serial dilutor, Journal of Industrial and Engineering Chemistry, 2020, 91, 231-239.
  2. Séverine Le Gac*, Marcia Ferraz, Bastien Venzac, Pierre Comizzoli*, Understanding and Assisting Reproduction in Wildlife Species using Microfluidics, Trends in Biotechnology, 2020, available online 7 October 2020. Invited review article.
  3. Carlo Alberto Paggi*, Bastien Venzac *&**, Marcel Karperien, Jeroen Leijten, Séverine Le Gac**, Monolithic microfluidic platform for exerting gradients of compression on cell-laden hydrogels, and application to a model of the articular cartilage, Sensors & Actuators B, 2020, 315, 127917.
  4. Valentina Palacio-Castadeña, Bastien Venzac*, Lucas Kooijman*, Wouter Verdurmen **, Séverine Le Gac **, Metabolic Switching of Tumor Cells under Hypoxic Conditions in a Tumor-on-a-chip Model, Micromachines, 2020, 11 (4), 382. Featured on the 10th anniversary of Micromachines.
  5. Linda Rikkert, Pepijn Beekman, Jaap Caro, Frank A. W. Coumans, Agustin Enciso Martinez, Guido Jenster, Séverine Le Gac, Woojee Lee, Ton van Leeuwen, G. B. Loozen, Afroditi Nanou, Rienk Nieuwland, Herman Offerhaus, Cess Otto,Michiel Pegtel Melissa Piontek, Edwin van der Pol, Leonie de Rond, W. H. Roos, Richard Schasoort, M Wauben, Han Zuilhof and Leon W. M. M. Terstappen, Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood, Frontiers in Oncology, 2020, 10, 608.
  6. Hoon Suk Rho, Henk-Willem Veltkamp, Danielle Baptista, J.G.E. Gardeniers, Séverine Le Gac*, Pamela Habibovic*, A 3D Polydimethylsiloxane Microhourglass-Shaped Channel Array Made by Reflowing Photoresist Structures for Engineering a Blood Capillary Network, Methods, 2020, in press.
  7. Dilu George Mathew*, Pepijn Beekman*, Serge Lemay, Han Zuilhof, Séverine Le Gac*, and Wilfred van der Wiel*, Electrochemical detection of tumor-derived extracellular vesicles on nanointerdigitated electrodes, NanoLetters, 2020, 202, 820-828. Cover article.
  8. Marcia de Almeida Monteiro Melo Ferraz, Jennifer Beth Nagashima, Bastien Venzac, Séverine Le Gac & Nucharin Songsasen, A dog oviduct-on-a-chip model of serous tubal intraepithelial carcinoma, Sci. Rep., 2020, 10, 1575.
  9. Marcia de Almeida Monteiro Melo Ferraz, Jennifer Beth Nagashima, Bastien Venzac, Séverine Le Gac & Nucharin Songsasen, 3D printed mold leachates in PDMS microfluidic devices, Sci. Repr., 2020, 10, 994.
  10. Swati Sharma*, Bastien Venzac*, Thomas Burgers, Séverine Le Gac^, Stefan Schlatt^, Microfluidics in male reproduction: Is ex vivo culture of primate testis tissue a future strategy for assisted reproductive technologies or toxicology research?, Molecular Human Reproduction, 26, 179-192. Invited “New Research Horizon” review.
  11. Silke Roovers, J. Deprez, Dwi Priwitaningrum, Guillaume Lajoinie, Nicolas Rivron, H Leclercq, O de Wever, Eleanor Stride, Séverine Le Gac, Michel Versluis, Jai Prakash, S De Smedt and Ine Lentacker,  Sonoprinting liposomes in tumor spheroids by micro bubbles and ultrasound, J. Control. Release, 2019, 316, 79-92.
  12. Renée M. Ripken, J. A. Wood, Han J. G. E. Gardeniers, Séverine Le GacAqueous-Phase Reforming in a Microreactor: The Role of Surface Bubbles, Chem. Eng. & Technol., 2019, 42, 2179-2186.
  13. Pepijn Beekman*, Agustin Encisco Martinez*, Hoon Suk Rho, Sidharam Pundlik PujariAufried Lenferink, Han Zuilhof, Leon Terstappen, Cees Otto* and Séverine Le Gac*, Immuno-capture of extracellular vesicles for individual multi-modal characterization using AFM, SEM and Raman spectroscopy, Lab Chip, 2019, 19, 2526-2536, Cover article.
  14. Renée M. Ripken, S. Schlauchtmann, Remco Sanders, Han J. G. E. Gardeniers, Séverine Le Gac, Monitoring Phase Behavior of Aqueous Biomass Model Substrates by High-Pressure and High-Temperature Microfluidics, Electrophoresis, 2019, 40, 563-570.
  15. Marcia de Almeida Monteiro Melo Ferraz, Hoon Suk Rho, Daiana Hemerich, Heiko Henning, Leni van Tol, Michael Hoelker, Urban Besenfelder, Michal Mokry, Peter Vos, Tom Stout, Séverine Le Gac, Bart Gadella, An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming, Nat. Comm., 2018, 9, 4934.
  16. Tom Kamperman, Marcel Karperien, Séverine Le Gac, Jeroen Leijten, Single-Cell Microgels: Technology, Challenges, and Applications, Tr. Biotech., 2018, 36, pp. 850. Cover article.
  17. Marcia Ferraz, Heiko Henning, Pedro Ferreira da Costa, Jos Malda, Séverine Le Gac, Fabrice Bray, Marjorie van Duursen, Jos Brouwers, Chris van de Lest, Ingeborg Bertijn, Lisa Kraneburg, Peter Vos, Tom Stout, Barend Gadella, Potential Health and Environmental Risks of Three-Dimensional Engineered Polymers, Env. Technol. Letters, 2018, 5, 80-85.
  18. Verena Schulze Greiving, Johan Bomer, Hans de Boer, Albert van den Berg, Séverine Le Gac, Integrated microfluidic biosensing platform for simultaneous confocal microscopy and electrophysiological measurements on bilayer lipid membranes and ion channels, Electrophoresis, 39, 496-503.
  19. Yoonsun Yang, Séverine Le Gac, Leon Terstappen, Hoon Suk Rho, Parallel Probing of Drug Uptake of Single Cancer Cells on a Microfluidic Device, Electrophoresis, 39, 548-556. Back cover article.
  20. Renée M. Ripken, Jan Meuldijk, Han (J. G. E.) Gardeniers and Séverine Le Gac, Influence of the water the stated on the thermodynamics of aqueous-phase reforming for hydrogen production, Chem. Sus. Chem., 2017, 24, 4909-4913.
  21. Fleur van Rossem, Johan Bomer, Hans de Boer, Yawar Abbas, Eddy de Weerd, Albert van den Berg, Séverine Le GacSensing oxygen at the millisecond time-scale using an ultra-microelectrode array (UMEA), Sens. Act. B, 2017, 238, 1008-1016.
  22. María Virumbrales-Muñoz, José María Ayuso, Marta Olave, Rosa Monge, Diego de Miguel, Luis Martínez-Lostao, Séverine Le Gac, Manuel Doblare, Ignacio Ochoa and Luis J. Fernandez, Multiwell capillarity-based microfluidic device for the study of 3D tumour tissue-2D endothelium interactions and drug screening in co-culture models, Sci. Rep., 2017, 7, 11998.
  23. Séverine Le Gac and Verena Nordhoff, Microfluidics for mammalian embryo culture and selection: where do we stand now?, Molecular Human Reproduction, 2017, 23, 213-226. Invited “New Research Horizon” review.
  24. Dwi L. Priwitaningrum, Jean-Baptiste G. Blondé, Adithya Sridhar, Joop van Baarlen,Wim E. Henning, Gert Storm, Séverine Le Gac, Jai Prakash, Tumor stroma-containing 3D spheroid arrays: A tool to study nanoparticle penetration, J. Control. Release, 2016, 244, 257-268.
  25. Nathalie Picollet-D’hahan, Monika E. Dolega, Lavinia Liguori, Christophe Marquette, Séverine Le Gac, Xavier Gidrol, Donald K. Martin, A 3D toolbox to enhance physiological relevance of human tissue models, Tr. Biotech, 2016, 34, 757-769.
  26. Ravi Sinha, Séverine Le Gac, Nico Verdonschot, Albert van den Berg, Bart Koopmans, Jeroen Rouwkema, Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations, Sci. Rep., 2016, 6, 29510.
  27. Zahra DembahriSéverine Le Gac, Kamal Tobal, Naziha Chirani , Christian Rolando, Farida Benmouna, Mustapha Benmouna, Polymer phase transition in n-lauryl methacrylate monoliths, Polym. Int., 2016, 65, 706-712.
  28. Jasper van Weerd, Shrikrishnan Sankaran, Oliver Roling, Sertan Sukas, Sven Krabbenborg, Jurriaan Huskens, Séverine Le Gac, Bart-Jan Ravoo, Marcel Karperien, Pascal Jonkheijm, A microfluidic device with continuous ligand gradients in supported lipid bilayers to probe effects of ligand surface density and solution shear stress on pathogen adhesion, Adv. Mat. Inter., 2016, 3, 1600055.
  29. Guillaume Lajoinie, Ine de Cock, Constantin C. Coussios, Ine Lentacker, Séverine Le Gac, Eleanor Stride, Michel Versluis, In vitro methods to study bubble-cell interactions: fundamentals and therapeutic applications, Biomicrofluidics, 2016, 10, 011501.
  30. Sarah Aβhoff, Sertan Sukas, Tadatsugu Yamaguchi, Catharina A. Hommersom, Séverine Le Gac*, Nathalie Katsonis*, Superstructures of chiral nematic microspheres as all-optical switchable distributors of light, Sci. Rep., 2015, 5, 14183.
  31. Hedwig M. Braakhuis, Samantha K. Kloet, Sanja Kezic, Frieke Kuper, Margriet V.D.Z. Park, Susann Bellmann, Meike van der Zande, Séverine Le Gac, Petra Krystek, Ruud J.B. Peters, Ivonne M.C.M. Rietjens, Hans Bouwmeester, Progress and future of in vitro models to study translocation of nanoparticles, Archiv. Toxicol., 2015, 89, 1469-1495.
  32. Dorit C. Kieslinger*, Zhenxia Hao*, Carlijn G. Vergouw, Elizabeth H. Kostelijk, Cornelis B. Lambalk, Séverine Le Gac, In vitro development of donated frozen thawed human embryos in a prototype static microfluidic device: a randomized controlled trial, Fertil. Steril., 2015, 103, 680-686.
  33. Ravi Sinha, Séverine Le Gac, Nico Verdonschot, Albert van den Berg, Bart Koopman, Jeroen Rouwkema, A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells, Lab Chip, 2015, 15, 429-435.
  34. Class-Willem Visser*, Marise V. Gielen*, Zhenxia Hao, Séverine Le Gac, Detlef Lohse, Chao Sun, Quantifying cell adhesion through impingement of a controlled microjet, Biophys. J., 2015, 108, 23-31. Highlighted in the Biophys. J. Newsletter. Click here for a movie.
  35. Björn Harink, Séverine Le Gac, David Barata, Clemens van Blitterswijk, Pamela Habibovic, Microfluidic platform with four orthogonal and overlapping gradients for soluble compound screening for use in regenerative medicine research, Electrophoresis, 2015, 36, 475-484..
  36. Johan Bomer, Alexander Prokofyev, Albert van den Berg, Séverine Le Gac, Wafer-scale fabrication of glass-FEP-glass microfluidic devices for lipid bilayer experimentation, Lab Chip, 2014, 14, 4461-4464.
  37. Yoonsun Yang, Joost Swennenhuis, Hoon Suk-Rho, Séverine Le Gac, Leon Terstappen, Parallel Single Cancer Cell Whole Genome Amplification Using Button-Valve Assisted Mixing In Nanoliter Chambers, PLOS ONE, 2014, 9, e107958.
  38. Adithya Sridhar, Albert van den Berg, Séverine Le Gac, Non-invasive monitoring of osteogenic differentiation on microtissue arrays under physiological conditions using scanning electrochemical microscopy, Electroanalysis, 2014, 26, 1881–1885.
  39. Sertan Sukas, Erik Schreuder, Björn de Wagenaar, Joost Swennenhuis, Albert van den Berg, Leon Terstappen, Séverine Le Gac, Novel side electrode configuration integrated in fused silica microsystems for synchronous optical and electrical spectroscopy, Lab Chip, 2014, 14, 1821-1825.
  40. Björn Harink, Séverine Le Gac, David Barata, Clemens van Blitterswijk, Pamela Habibovic, Microtiter plate-sized standalone chip holder for microenvironmental physiological control in gas-impermeable microfluidic devices, Lab Chip, 2014, 7, 1816-1820.
  41. Adithya Sridhar, Hans L. de Boer, Albert van den Berg, Séverine Le Gac, Microstamped Petri dishes for scanning electrochemical microscopy analysis of arrays of microtissues, PLOS ONE, 2014, 9, e93618.
  42. Sandra A. Henkemeyer, Caroline Schwarzer, Michele Boiani, Jens Ehmcke, Séverine Le Gac, Stefan Schlatt, Verena Nordhoff, Effects of embryo culture media do not persist after implantation: a histological study in mice, Hum. Reprod., 2014, 29, 220-233.
  43. Telma C. Esteves, Fleur van Rossem, Verena Nordhoff, Stefan Schlatt, Michele Boiani, Séverine Le Gac, Microfluidic system supports single mouse embryo culture leading to full-term development, RSC Adv., 2013, 3, 26451-26458.
  44. Björn Harink, Séverine Le Gac, Roman Truckenmüller, Clemens van Blitterswijk, Pamela Habibovic, Regeneration-on-a-chip? The perspectives on use of microfluidics in regenerative medicine, Lab Chip, 2013, 13, 3512–3528. Invited review article
  45. Verena Stimberg, Johan G. Bomer, Iris van Uitert, Albert van den Berg, Séverine Le Gac, High-yield, reproducible and quasi-automated bilayer formation in a microfluidic format, Small, 2013, 9, 1076-1085Cover article.
  46. Toyohiro Naito, Rerngchai Arayanarakool, Séverine Le Gac, Takao Yasui, Noritada Kaji, Manebu Tokeshi, Albert van den Berg, Yoshinobu Baba, Temperature-driven self-actuated microchamber sealing system for highly integrated microfluidic devices, Lab Chip, 2013, 13, 452-458.
  47. Caroline Schwarzer, Telma C. Esteves, Marcos J. Arauzo-Bravo, Séverine Le Gac, Verena Nordhoff, Stefan Schlatt, Michele Boiani; ART culture conditions change the probability of mouse embryo gestation through defined cellular and molecular responses, Human Reprod., 2012, 27, 2627-2640. Cover article.
  48. Nicolas C. Rivron, Erik J. Vrij, Jeroen Rouwkema, Séverine Le Gac, Albert van den Berg, Roman Truckenmüller, Clemens A. van Blitterswijk, Tissue deformation spatially modulates VEGF signaling and angiogenesis. PNAS, 2012, 109, 18, 6881-6891.
  49. Floris van den Brink, Elmar Gool, Jean-Philippe Frimat, Johan Bomer, Albert van den Berg, Séverine Le Gac, Parallel single cell analysis (PaSCAl) microfluidic platform, Electrophoresis, 2011, 32, 3094–3100. Cover article.
  50. Iris van Uitert, Séverine Le Gac, Albert van den Berg, Determination of the electroporation onset of bilayer lipid membranes as a novel approach to establish ternary phase diagrams: example of the l-a-PC/SM/cholesterol system, Soft Matter, 2010, 6, 4420-4429.
  51. Rerngchai Arayanarakool, Séverine Le Gac, Albert van den Berg, Low temperature, simple and fast integration technique of microfluidic chips using a UV-curable adhesive, Lab Chip, 2010, 10, 2115-2121.
  52. Séverine Le Gac, Albert van den Berg, Single cells as experimentation units in lab-on-a-chip devices, Tr. Biotech., 2010, 28, 55. Invited opinion article.
  53. Iris van Uitert, Séverine Le Gac, Albert van den Berg, Towards a better understanding of the mechanism of (electro)pore formation using planar membrane models, Biophysica Biochimica Acta membranes, 2010, 1798, 21-31.
  54. Erwin Nijhuis, Séverine Le Gac, Andre A. Poot, Jan Feijen, Istvan Vermes, Bax-mediated mitochondrial membrane permeabilization after heat treatment is caspase-2 dependent, Int. J. Hyperthermia, 2008, 24, 357. Rory Dijkink, Séverine Le Gac, Erwin Nijhuis, Albert van den Berg, Istvan Vermes, Claus-Dieter Ohl, Controlled cavitation–cell interaction: trans-membrane transport and viability studies, Phys. Med. Biol., 2008, 53, 375.
  55. Rory Dijkink, Séverine Le Gac, Erwin Nijhuis, Albert van den Berg, Istvan Vermes, Claus-Dieter Ohl, Laser-induced cavitation bubbles for drug deliver into biological cells, Phys. Med. Biol., 2008, 53, 375-390.
  56. Séverine Le Gac, Ed Zwaan, Albert van den Berg, Claus-Dieter Ohl, Sonoporation of suspension cells with single cavitation bubbles in a microfluidic environment, Lab Chip, 2007, 7, 1666.
  57. Ed Zwaan, Séverine Le Gac, Kinko Tsuji, Claus-Dieter Ohl, Controlled cavitation in microfluidic systems, Phys. Rev. Let., 2007, 98, 254501.
  58. Floor Wolbers, Paul ter Braak, Séverine Le Gac, Regina Luttge, Helene Andersson, Istvan Vermes, Albert van den Berg, Viability study of HL60 cells in contact with commonly used microchip materials, Electrophoresis, 2006, 27, 5073-5080.
  59. Séverine Le Gac, Istvan Vermes, Albert van den Berg, Quantum dots-based probes conjugated to Annexin V for photostable apoptosis detection and imaging, Nano Lett., 2006, 6, 1863-1869.
  60. Séverine Le Gac, Steve Arscott, Christian Rolando, An open design microfabricated nib-like nanoelectrospray emitter tip on a conducting silicon substrate for the application of the ionization voltage, J. Am. Soc. Mass Spectrom., 2006, 17, 75-80. Cover article.
  61. Julien Carlier, Steve Arscott, Vincent Thomy, Jean-Christophe Camart, Cécile Cren-Olivé, Séverine Le Gac, Integrated microfabricated systems including a purification module and an on-chip nano electrospray ionization interface for biological analysis, J. Chrom. A, 2005, 1071, 213-222.
  62. Steve Arscott, Séverine Le Gac, Christian Druon, Pierre Tabourier, Christian Rolando, Polysilicon nanoelectrospray emitter tips for mass spectrometry, Sens. and Act. B, 2005, 106, 241-249.
  63. Martin Brinkmann, Steve Arscott, Séverine Le Gac, Christian Druon, Pierre Tabourier, Christian Rolando, Ralf Blossey, Microfluidic design rules for capillary-slot based electrospray sources, Appl. Phys. Lett., 2004, 85, 2140-2142.
  64. Séverine Le Gac, Julien Carlier, Cécile Cren-Olivé, Jean-Christophe Camart, Christian Rolando, Monoliths for microfluidics devices in proteomics, J. Chrom. B, 2004, 808, 3-14.
  65. Steve Arscott, Séverine Le Gac, Christian Druon, Pierre Tabourier, Christian Rolando, A micromachined 2D nanoelectrospray emitter, Elec. lett., 2003, 39, 1702-1703.
  66. Steve Arscott, Séverine Le Gac, Christian Druon, Pierre Tabourier, Christian Rolando, A Micro-nib nanoelectrospray source for mass spectrometry, Sens. and Act. B, 2004, 98, 140-147.
  67. Séverine Le Gac, Steve Arscott, Cécile Cren-Olivé, Christian Rolando, A novel nib-like design for microfabricated nanospray tips, J. Am. Soc. Mass Spectrom., 2004, 15, 409-412.
  68. Steve Arscott, Séverine Le Gac, Christian Druon, Pierre Tabourier, Christian Rolando, A planar on-chip micro-nib interface for NanoESI-MS microfluidic applications, J. Micromech. Microeng., 2004, 14, 310-316.
  69. Séverine Le Gac, Steve Arscott, Christian Rolando, Two-dimensional microfabricated sources for nanoelectrospray, J. Mass Spectrom., 2003, 38, 1259-1264.
  70. Séverine Le Gac, Steve Arscott, Christian Rolando, A planar microfabricated nanoelectrospray tip based on a capillary slot, Electrophoresis, 2003, 24, 3640- 3647.

National (refereed) journals

  1. Mohammed Kajjout, Christian Rolando, Séverine Le Gac, Microfluidics reactors and selectivity for organic chemistry applications, La Houille Blanche-Revue internationale de l’eau, 2006, 2, 83-86.
  2. Julien Carlier, Steve Arscott, François Caron, Jean-Christophe Fourrier, Vincent Thomy, Jean-Christophe Camart, Christian Druon, Pierre Tabourier, Kasia Chuda, Xavier Coqueret, Séverine Le Gac, Christian Rolando, Microfluidique intégrée pour le couplage d’un microcanal à une interface nanoESI : application à la protéomique, La Houille Blanche-Revue internationale de l’eau, 2006, 3, 60-64.
  3. Séverine Le Gac, Steve Arscott, Christian Rolando, Novel microfabricated nanoESI sources for proteomics applications, La Houille Blanche-Revue internationale de l’eau, 2006, 5, 45-50.
  4. Séverine Le Gac, Christian Rolando, Christian Druon, Pierre Tabourier, Microfluidic system for proteomics analysis by ESI-MS, La Houille Blanche-Revue internationale de l’eau, 2003, 3, 60-64.

Wide-audience articles

  1. Séverine Le Gac, Steve Arscott, Christian Rolando, Nouvelles sources de nano-ESI microfabriquées dédiées à la protéomique, Spectra Analyse, 34, 2005, 20-25.
  2. Séverine Le Gac, Christian Rolando, Protéomique et microfluidique: le consortium BioChipLab, Biofutur, Novembre 2004. Special issue Nanobio(techno)logy.
  3. Séverine Le Gac, Christian Rolando, Microsystèmes d’analyse biologique, Chimix (Sigma-Aldrich), 9, 2003, 2-3.
  4. Séverine Le Gac, Christian Rolando, Les microsystèmes en chimie, L’actualité chimique, February 2002, 21-31. Review.